mouse leydig tumor cells mltc 1  (ATCC)

Journal: International journal of molecular sciences

Article Title: Highly-Sensitive In Vitro Bioassays for FSH, TSH, PTH, Kp, and OT in Addition to LH in Mouse Leydig Tumor Cell.

doi: 10.3390/ijms241512047

Figure Lengend Snippet: Figure 1. Effect of 1/10 diluted sera from various species or BSA on the cAMP response to ovine LH. (A) Effect of sera. Using the standard protocol including forskolin and oxytocin [1] in the mLTC medium, the sera from various species were present at 1% final concentration. (B) Effect of BSA (0 or 1 mg/mL) during oLH dilution (BSA dil) compared to the same final BSA quantity added separately to the cell medium (BSA cell). AUC: Area Under 60 min kinetics Curves.

Article Snippet: The mouse Leydig Tumor Cells (mLTC) were obtained from the American Tissue and Cell Collection (ATCC) (LGC Standards, Molsheim, France) and have been regularly tested negative for mycoplasma contamination all over our studies.

Techniques: Concentration Assay

Journal: International journal of molecular sciences

Article Title: Highly-Sensitive In Vitro Bioassays for FSH, TSH, PTH, Kp, and OT in Addition to LH in Mouse Leydig Tumor Cell.

doi: 10.3390/ijms241512047

Figure Lengend Snippet: Figure 2. Upper panel: Cyclic AMP response to hFSH (A) and hLH (B) after transfection of mLTC with increasing concentrations of hFSHR expression plasmid. The mLTC cells were transfected with no or 6.25 to 100 ng hFSHR plasmid before challenging with 10–1000 pM concentrations hFSH (A) or hLH (B). (C) hFSH dose–response curves when the hormone is diluted in RPMI medium alone (blue dots) or completed with 1 mg/mL BSA (red dots).

Article Snippet: The mouse Leydig Tumor Cells (mLTC) were obtained from the American Tissue and Cell Collection (ATCC) (LGC Standards, Molsheim, France) and have been regularly tested negative for mycoplasma contamination all over our studies.

Techniques: Transfection, Expressing, Plasmid Preparation

Journal: International journal of molecular sciences

Article Title: Highly-Sensitive In Vitro Bioassays for FSH, TSH, PTH, Kp, and OT in Addition to LH in Mouse Leydig Tumor Cell.

doi: 10.3390/ijms241512047

Figure Lengend Snippet: Figure 3. Cyclic AMP response to ovine TSH in mLTC transfected with the oTSHR. (A): oTSH dose–response curves in the absence or presence of 1/20 ovine serum (0.5% final concentration). (B): oTSH dose–response curves when the hormone is diluted in RPMI medium alone (blue dots) or completed with 1 mg/mL BSA (green dots). (C): oTSH concentration profiles in serums at 20-fold dilution measured by the in vitro bioassay compared to oTSH RIA. The red curve shows the AUC in the absence of TSHR (thus due only to oLH in the samples).

Article Snippet: The mouse Leydig Tumor Cells (mLTC) were obtained from the American Tissue and Cell Collection (ATCC) (LGC Standards, Molsheim, France) and have been regularly tested negative for mycoplasma contamination all over our studies.

Techniques: Transfection, Concentration Assay, In Vitro, Bioassay

Journal: International journal of molecular sciences

Article Title: Highly-Sensitive In Vitro Bioassays for FSH, TSH, PTH, Kp, and OT in Addition to LH in Mouse Leydig Tumor Cell.

doi: 10.3390/ijms241512047

Figure Lengend Snippet: Figure 4. Dose-dependent cyclic AMP response to 10−14–10−8 M hPTH in mLTC transfected with hPTHR. The mLTC cells were transfected with 0 or 1.56 to 12.5 ng PTHR plasmid per well before challenging with 10−14–10−8 M hPTH 1–34.

Article Snippet: The mouse Leydig Tumor Cells (mLTC) were obtained from the American Tissue and Cell Collection (ATCC) (LGC Standards, Molsheim, France) and have been regularly tested negative for mycoplasma contamination all over our studies.

Techniques: Transfection, Plasmid Preparation

Journal: International journal of molecular sciences

Article Title: Highly-Sensitive In Vitro Bioassays for FSH, TSH, PTH, Kp, and OT in Addition to LH in Mouse Leydig Tumor Cell.

doi: 10.3390/ijms241512047

Figure Lengend Snippet: Figure 6. Dose-dependent cyclic AMP response to 10−12–10−7 M kisspeptin variants together with 0.3 nM oLH. The mLTC were transfected with 100 ng/well of the GPR54 hKp receptor plasmid before challenging with Kp and oLH added together at t48h for luminescence recording.

Article Snippet: The mouse Leydig Tumor Cells (mLTC) were obtained from the American Tissue and Cell Collection (ATCC) (LGC Standards, Molsheim, France) and have been regularly tested negative for mycoplasma contamination all over our studies.

Techniques: Transfection, Plasmid Preparation

Journal: International journal of molecular sciences

Article Title: Highly-Sensitive In Vitro Bioassays for FSH, TSH, PTH, Kp, and OT in Addition to LH in Mouse Leydig Tumor Cell.

doi: 10.3390/ijms241512047

Figure Lengend Snippet: Figure 8. Effect of OT (A) or AVP (B) on the cyclic AMP response to hCG in mLTC. The mLTC cells were pre-incubated for 2 h with the shown concentrations of 10−20–10−6 M OT (A) or AVP (B) before stimulation with 2.5 × 10−10 M hCG and luminescence recording.

Article Snippet: The mouse Leydig Tumor Cells (mLTC) were obtained from the American Tissue and Cell Collection (ATCC) (LGC Standards, Molsheim, France) and have been regularly tested negative for mycoplasma contamination all over our studies.

Techniques: Incubation